Optical coherence tomography (OCT) is capable of 2–3mm slicing in opaque samples and so provides real-time cross-sectional images from the specimen. OCT can also achieve resolutions approaching that of conventional histology (c. 2–5μm) as it employs very short wave near-infrared light with precise interferometric detection. Although now mainly used in ophthalmology, OCT has already been found to have clinical applications in dermatology, cardiology, neurology, gynecology and gastroenterology. Dr. Ronan Cahill have deployed a commercially available probe (NIRIS™, Imulax Corporation, Cleveland OH, USA) via the working channel of a gastroscope used as a N.O.T.E.S. peritoneoscope in an experimental model. By this means, high-resolution real-time images mesenteric sentinel nodes have been obtained. Placing such a probe on the surface of the node should provide examination of the tissue to a depth of 2mm. In this video, we show the application of this technique in an experimental model.

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